《一仆二主》講述的是人到中年的楊樹是單身女老板唐紅的私人司機，帶著18歲的女兒楊樹苗，青春期的她任性強硬，和楊樹衝突不斷。唐紅一直沒有結婚，雖然身邊有土老板孟來財追求，但她愛著楊樹，對楊樹苗也關愛有加。礙於身份的考慮，正直老實的楊樹一直不願意接受唐紅的愛意。在女兒設計下，楊樹結識了頗像前妻的年輕女孩顧菁菁，兩人互生好感。楊樹就這樣夾在了唐紅和顧菁菁之間，一幕幕喜劇故事上演。後來，前妻的回國，讓楊樹放下了對前妻的執念，在與唐紅的相處中楊樹看清自己的感情，勇敢面對她的愛，兩人最終走到一起；顧菁菁在與孟來財相識後，惺惺相惜的二人也產生真摯感情，最終四人各自情歸所屬。

　　杨树（张嘉译 饰）人到中年，独自抚养着十八岁的女儿杨树苗（关晓彤 饰），树苗正是叛逆的年龄，张扬不羁的她和保守老实的杨树之间产生了诸多的矛盾。杨树是女老板唐红（闫妮 饰）的司机，尽管唐红才貌双全，但个性清高的她至今依旧单身。随着时间的推移，唐红渐渐爱上了踏实善良的杨树，但身份的巨大差异成为了坚实的屏蔽横亘在两人之间。

　　在树苗的撮合之下，杨树结识了名叫顾菁菁（江疏影 饰）的年轻女子，顾菁菁对杨树一见倾心，杨树也因为顾菁菁和自己的前妻十分相像而对其心生好感。眼看着顾菁菁和杨树之间的距离越走越近，唐红的心里很不是滋味。

Yang Shu (Zhang Jiayi) works as a personal driver for Tang Hong (Yan Ni) who is an accomplished business woman. His daughter Yang Shumiao (Guan Xiaotong) is stubborn and rebellious which results in frequent arguments between the two.

Even though Meng Laicai (You Yong) has been pursuing her, Tang Hong hasn't married in all these years because she is in love with Yang Shu. However, their difference in status has prevented Yang Shu from accepting Tang Hong. Through his daughter, Yang Shu meets Gu Qingqing (Maggie Jiang), a young woman who resembles his ex-wife. As Yang Shu gets caught between two women, it ignites a series of events.