Jun has overcome his past and must now make up all his schoolwork before he can actually go back. Suiseiseki and Souseiseki barge into the Sakurada residence as usual, the dolls play with each other, and everything is back to normal. However, Shinku starts to face deep nightmares about Suigintou. Barasuishou, the fraud of the seventh doll of the Rozen Maiden, is introduced.

Shinku claims that Barasuishou never meet any of the dolls of the Rozen Maiden. Since Barasuishou is the seventh doll, this appears to be a sign that the Alice Game would be ending soon. Shinku's strange behavior continues, and Jun continues to worry about her. Jun and Tomoe also visit the doll shop of a doll maker named Enju.

Kanaria, the second doll of the Rozen Maiden, is introduced. Her initial plan is to sneak into the Sakurada residence and take the Rosa Mysticae from Shinku, Hinaichigo, Suiseiseki, and Souseiseki. However, chaos erupts as the dolls think that a burglar broke in.

Suiseiseki decides to find a medium, as Jun is the only one eligible, though she is skeptical about him. Ultimately, Jun and the five dolls are taken to the world of Barasuishou, where the Alice Game is foreshadowed. Jun enters into a contract with Suiseiseki.

Hinaichigo tries to send a letter to Jun. Suiseiseki tries to earn Jun's respect. Souseiseki tries to keep order in the house Kanaria tries to get into the Sakurada residence yet again. Jun and Shinku are in the background during these events.

Megu, hospitalized with an incurable disease, enters into a contract with Suigintou. However, Megu wishes only for death and insists that she is incomplete and broken. After encountering Barasuishou, and later Shinku, it is realized that Suigintou was revived to participate in the Alice Game.

After having seen an apparition of Rozen, the creator of the dolls of the Rozen Maiden, Souseiseki soon sees the deep meaning behind the Alice Game. After having seen Enju and his feelings about dolls, Jun starts to wonder more about Rozen and his love for his dolls. Souseiseki resolves to fight her sisters, take their Rosa Mysticae, and become Alice.

Suiseiseki becomes worried about Souseiseki. Souseiseki battles against Barasuishou and Suigintou, only to be defeated. Souseiseki asks Suiseiseki to take her Rosa Mystica, but Suiseiseki fails and Suigintou takes Souseiseki's Rosa Mystica.

Souseiseki is destined to meet a tragic fate, and to make matters worse, Shinku sees the same for Hinaichigo as well. Hinaichigo is also aware of her destiny, and shares her last moments with Tomoe. There is no time to mourn, however, since Barasuishou, Laplace, and Enju have apparently laid a trap for Jun and the remaining dolls of the Rozen Maiden.

Because they have yet to fulfill Rozen's wish, the remaining dolls fight Barasuishou and Suigintou. When things spiral downward, Shinku is left to fight Barasuishou.

The conflicts are solved, the battles are ended. Jun learns the shocking truth about Barasuishou, Enju, and Rozen.