Muffin the Mule is a puppet character in British television programmes for children. The original programmes featuring the character were presented by Annette Mills, sister of John Mills, and broadcast live by the BBC from their studios at Alexandra Palace from 1946 to 1952. Mills and the puppet continued with programmes that were broadcast until 1955, when Mills died. The series then transferred to ITV in 1956 and 1957. A modern animated version of Muffin appeared on the BBC in 2005.

The original mule puppet was created in 1933 by Punch and Judy puppet maker Fred Tickner for husband-and-wife puppeteers Jan Bussell and Ann Hogarth to form part of a puppet circus for the Hogarth Puppet Theatre. The act was soon put away, and the puppet was not taken out again until 1946, when Bussell and Hogarth were working with presenter Annette Mills. Shes named the puppet mule "Muffin", and it first appeared on television in an edition of For The Children broadcast on 20 October 1946.