When the Backyardigans receive a strange signal originating from Mars, Uniqua, Pablo and Austin blast off to investigate. They're backed up by Tyrone and Tasha, who remain on Earth to assist at Mission Control. The Backyardigans leave prepared for anything, but encounter some surprises along the way. They don't give up, however, and eventually make a surprising discovery regarding the source of the signal.

Pie makers Tyrone and Austin must make a pie for Empress Tasha, while protecting it from Ninjas Uniqua and Pablo.

Sherlock Holmes style detective, Pablo, travels to Mystery Manor to find out who stole Lady Tasha's jewels. Among the suspects are Butler Tyrone, Mr. Austin Frothingslosh, and Inspector Uniqua Underhood.

In order to stop the volcano from ruining their luau, the Luau Brothers (Austin, Pablo and Tyrone) must give the Volcano Sisters (Tasha and Uniqua) a special gift.

Swamp Tour Guides Uniqua and Pablo promise to show tourists Tasha and Austin the Swamp Creature, even if they have to trick them by pretending to be the creature themselves. Little do they know what lurks deep in the swamp.

Mad Scientist Tasha sends Austin to bring three monsters back to her laboratory. They are looking for Vampire Pablo, Mummy Tyrone, and Werewolf Uniqua.

Uniqua set off in search for the Ice Lady, Tasha, because there is no snow yet. Along the way she gets help from Cowboy Pablo, Tarzan Tyrone, and Austin, the Ice Lady's assistant.

Tyrone, a mailman, becomes scared by the "Do Gooders," a motorcycle gang formed by Tasha, Uniqua and Pablo. While Tyrone believes they want to steal his mail, the gang is trying to make deliveries of their own.

Uniqua and Pablo are a cowgirl and a jockey. When they argue about who is a faster rider, they decide to settle the matter with a race to the end of the Mississippi River, with Austin serving as the announcer. To their surprise, Tyrone enters the race on a tired, old-looking donkey named Burro. As the race progresses, the two make a quick dash for the finish, but Tyrone and his donkey gain ground as the two continuously stop to argue who is better.

Movers Uniqua and Pablo are hired by the new Sultan of Arabia, to safely move his magical treasures from a cave in the desert to his new royal palace. Can they complete the job without using all of the genie's three wishes?

Tasha and Pablo are a couple of bad robots who are out to destroy all of the worlds good robots and reprogram them to become bad.

Tasha is a fisherlady who has a knack for navigating into dangerous areas of the ocean. Pablo, Tyrone and Uniqua are part of a rescue squad, determined to save her. Tasha, on the other hand, is determined to catch a whopper and tries to rebuff their efforts to help.

Sinbad (Tyrone) is sailing to find the end of the rainbow. Pablo, a young, inexperienced sailor (and a huge fan of Sinbad), sneaks aboard to help him. When Sinbad discovers this, he is upset, as he has always sailed alone and doesn't feel he need any help. Indeed, at first, Pablo seems like only a bother, causing the loss of ship's supplies, forcing them to take detours where they must play games to earn new supplies. Pablo, however, refuses to be daunted and searches for a way that he can help his idol.

Pablo and Tyrone are deep sea explorers who are looking for a mermaid to take pictures of. They find two, Tasha and Uniqua, who think they are being invaded and are going to be captured.

Miss T (Tasha), who is the head of the International Super Spy agency, orders Agent Secret (Pablo) to stop the villainous Lady in Pink (Uniqua) from stealing three top-secret containers. With the help of his undercover contact Austin, Agent Secret travels around the world to a exotic locations in London, Switzerland, Miami and the Caribbean to collect the three containers before the Lady in Pink and her evil henchman Tyrone, get a chance to steal them.

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Uniqua and Tyrone are royal knights who are protecting King Pablo's egg. Grabbing Goblin Austin in Goblin Land and the Fairy Tasha want the egg for themselves. They follow King Pablo's egg to Dragon Mountain to retrieve it.

Knights Uniqua and Tyrone lose track of their charge, the king's special egg, and scour the countryside hoping to recover the precious item. Along the way they run into a goblin and a fairy, who don't exactly lend a hand in the search, but insist on tagging along. Audioslave writes and performs the new original song "I'm Not An Egg Anymore."

Professor Pablo is bound and determined to find the rarest specimen, the gilded butterfly. While he is out searching for it, he believes he stumbles upon it, and hires rugged stagecoach drivers Tyrone and Uniqua to help hunt in down in the Old West.

Queen Uniqua sends wizards, Tyrone and Pablo, out to get rid of Tasha, the giant. Tasha is new to the kingdom and is just looking for fun and new friends, but Queen Uniqua would rather take a nap. Before long, Tyrone and Pablo use a magic spell and manage to accidently turn everyone into giants.

Tasha, Uniqua and Austin work for W-I-O-WA News (The Corniest Station in the Nation), which is a news station that usually ends up reporting on corn. Tyrone and Pablo are corn farmers who are so happy with their crop, that they keep sending in reports just to get on the air, but unfortunately it isn't considered newsworthy. They decide to make up some stories about aliens visiting their cornfields so that the news team will come out to investigate further and put their corn crops on their air.