Saturday Night Live aired its sixteenth season during the 1990-1991 television season on NBC. 20 episodes were produced. The sixteenth season began on September 29, 1990, and ended on May 18, 1991.

The 16th season of SNL was a transitional one: Several longtime cast members left, and a large number of additions were made to the roster. To ensure that he was not short on talent, Michaels chose to retain most of the late 1980s cast while in the process of hiring the people that would make up the early 1990s cast. At one point during the season, sixteen people were listed as cast members or featured players.