Doi frați, Sam și Dean Winchester, sunt martorii morții paranormale a mamei lor pe când erau copii și cresc antrenați în luptă de un tată dezorientat care nu dorește nimic mai mult decât să vâneze lucrul care i-a ucis soția. Sam evadează la facultate pentru a începe o viață nouă, normală, dar este atras înapoi după ce Dean îi apare la ușă pentru a-i spune că tatăl lor a dispărut.

Urmărind indiciile unui mesaj telefonic sinistru de la acesta, băieții călătoresc într-un orășel și întâlnesc un spirit răzbunător numit "Femeia în alb", care apoi începe să-l bântuie pe Sam.

Sam și Dean urmează coordonatele lăsate în jurnalul tatălui lor și ajung în mijlocul pădurii unde investighează dispariția mai multor rulote. Cei doi frați află curând că au de-a face cu un Wendigo, o creatură devenită celebră în legendele nativilor din America. Un Wendigo este un fost om al cărui canibalism l-a transformat într-o creatură cu forță și viteză supraomenească, care se hrănește cu carne umană.

Sam și Dean investighează o serie de înecuri misterioase considerate sinucideri. Ei descoperă că spiritul este un băiețel furios, cu puterea de a călători prin apă, care se răzbună pe anumiți locuitori ai orașului pentru un secret întunecat îngropat cu mult timp în urmă. Dean formează o legătură cu un băiețel care pare să aibă puterea de premoniție.

La bordul zborului TransNational 2485, un bărbat posedat de spiritul călătorului fantomă provoacă prăbușirea avionului, lăsând în viață doar cinci supraviețuitori. Sam și Dean trebuie să exorcizeze spiritul furios înainte ca acesta să-i atace pe supraviețuitori. Frații își dau seama că singura modalitate de a face asta este să ducă lupta sus în cer.

În suburbia Nebraska, un grup de adolescenți petrecăreți din liceu îl provoacă în glumă pe prietenul lor Charlie să se privească în oglindă și să repete "Bloody Mary, Bloody Mary, Bloody Mary, Bloody Mary", fără să știe că acest lucru va declanșa o serie de crime misterioase. Sam și Dean își dau seama că Bloody Mary are puterea de a călători prin toate suprafețele care reflectă lumina atunci când vânează o victimă. Îngrijorați că adolescenții o invocă accidental pe Bloody Mary, Sam și Dean se întrec să distrugă spiritul violent înainte ca aceasta să poată ucide din nou.

Sam and Dean come to the aid of Sam's old college buddy after he is accused of a murder he swears he didn't commit. The brothers discover a shapeshifter is adopting the likenesses of others and murdering people. The brothers' race to stop the shapeshifter takes a terrifying turn when he shifts into the likeness of Dean.

The infamous "Hook Man," a vengeful spirit who kills his victims with a shiny hook that serves as his hand, terrorizes a small college town in Iowa. Sam and Dean learn that all the victims are connected to the daughter of a local minister and race to find and destroy the Hook Man's bones before he comes for them.

After a construction worker is killed by insects burrowing into his brain, Sam and Dean investigate the town's history and find that the new housing development is being built on sacred Indian land. The Indians put a curse on the land after their reservation had been ravished and destroyed. Sam and Dean must find a way to survive and kill the deadly swarm of bees, locusts, spiders and beetles.

Sam is haunted by a vision of a woman trapped in his childhood house and convinces a reluctant Dean that they need to go home. Upon arrival, they meet the woman in Sam's premonition and she reveals that the house is haunted. Looking into their personal history, the brothers discover their father had visited a psychic after their mother's death and they turn to the same woman for answers of their own. Together they discover a malevolent poltergeist has inhabited the house and set out to exorcise it, but are blindsided when another unseen spirit steps in.

Sam and Dean investigate an abandoned sanitarium and discover that when the hospital was open, the patients held a revolt against the cruel and unusual punishments inflicted by the head doctor. While the brothers search the premises for four lost college students, the tortured spirits cause them to go insane, turning Dean against Sam.

Sam and Dean finally make contact with their father, but he tells them to stop looking for him and sends them on another ghost-hunting job. Frustrated with their father's evasiveness, Sam sets out alone to find him while Dean stays back to obey his father. Dean investigates a small town in Indiana where couples have gone missing the same day each year and discovers the farmers are sacrificing them to a Pagan God who takes the form of a scarecrow.

While battling a demon, Dean is electrocuted, resulting in permanent damage to his heart and leaving him with only a couple of months to live. A despondent Sam searches desperately for a way to save his brother and believes he may have found an answer through a preacher who claims to heal the incurable. However, Dean and Sam discover that the preacher is getting help from the Grim Reaper who is trading one life for another he feels is less worthy.

Dean is contacted by his first love Cassie, an African-America girl, who asks him to come to Mississippi to investigate a string of racially motivated murders. Each murder is linked to a mysterious truck that seems to have no driver and leave no tracks. Sam is stunned to see this new side of his brother as Dean struggles to come to grips with the residual feelings he has for Cassie.

Sam has a premonition in which a man is killed, but the murder is made to look like a suicide. Sam convinces Dean that they must investigate the case, but the two are puzzled when they fail to find anything that indicates that the death was supernatural in nature. That is, until they find out that Max, the reclusive son of the first victim, has been using his recently acquired power of telekinesis to kill the family members that once abused him. Sam also discovers Max's mother was killed by the same fiery demon that took his own mom.

Sam and Dean head to Minnesota where a young boy witnesses a man vanish into thin air. Upon searching for clues, Sam is abducted by what he assumes is a supernatural being, and Dean is left to search frantically for his brother. However, both are stunned when they realize the kidnappers are human, and using the captives as human prey for a twisted hunting expedition.

Sam has a reunion with a girl he once met, Meg, but it turns out that she is using the brothers to lure their father John to his doom.

Sam and Dean battle a Shtriga, a witch that sneaks into childrens' bedrooms and sucks the lives out of them. Dean must relive his childhood when he remembers that he encountered the Shtriga years ago.

The Winchesters must deal with a dangerous spirit bound to an old family portrait that brings misfortune and death to anyone who buys it.

To obtain a mystic gun capable of destroying the demon that killed their mother, Sam and Dean must team up with their father John and face off against vampires.

John has realized where the demon will strike next and the Winchesters head there to stop the next killing. But Meg begins killing John's friends and demands the Colt or she'll keep going until everyone John knows is dead.

When Meg boasts that John is her prisoner, the boys visit a friend of their father's to get information about demonic possessions and ways to trap a demon. It turns out Meg is not what she appears.