这是五月天首次将自己的“变形 DNA 无限放大版”的全球巡回演唱会搬上了大银幕，并采用了3D技术。在这部以五月天演唱会为主线的电影里面，穿插了三个小故事：广州，一位经营咖喱鱼蛋的父亲，面对宝贝女儿疯狂的追星行为最初完全无法理解，由此激化了 父女之间的矛盾。不过父亲永远是爱女儿了，最终还是支持了女儿独自前往上海的追星之行。台北，一个被爱情深深伤害的出租车司机，却因为遇到了一位神秘的女乘客，在台北街头展开了一场奇异的旅程。短暂的旅途过后，两个陌生人的距离逐渐被拉近……上海，勤快的快递员每日辛苦工作，穿梭于上海的大街小巷。每天的所得，他都会拿出很大一部分存起来，就是为了能圆已逝妹妹的心愿，去看一场五月天的演唱会。

世界上最難追的票是五月天，一生中最難追的夢是自己……一碗魚蛋、一台單車、一輛小黃、一張演唱會門票的追夢之旅！五月天演出並全程參與故事發想、電影配樂等幕後製作，三段歌迷溫馨爆笑故事巧妙結合五月天在亞洲「變形DNA無限放大版」演唱會精華片段，以3D立體電影攝製技術創造新的電影形式。

Billed as a "concept film" MAYDAY 3DNA combines play from the group's DNA concert tour in Taiwan, Hong Kong, Singapore and China 2010 and interweaves that with three fictional stories. The fictional sequences include a vignette about a Guangzhou father and daughter; another about a Taiwan taxi driver and passenger; and a third about a Shanghai delivery boy. All are affected by the lure of a Mayday gathering and the three separate stories intersect at one particular Mayday concert in Shanghai. Established in Taiwan in the late 1990s, Mayday has remained popular for over a decade by tuning into a Taiwan youth beat and pushing rock music in Greater China. Most songs are performed in Mandarin with some Taiwanese Hokkien tracks by band.