Pemeran 16

1. 

   Josh Keaton

   Peter Parker/Spider-Man (voice)

2. 

   Lacey Chabert

   Gwen Stacy (voice)

3. 

   James Arnold Taylor

   Harry Osborn (voice)

4. 

   Alan Rachins

   Norman Osborn (voice)

5. 

   Deborah Strang

   Aunt May Parker (voice)

6. 

   Ben Diskin

   Eddie Brock (voice)

7. 

   Dee Bradley Baker

   Curt Connors/Lizard (voice)

8. 

   Robert Englund

   Adrian Toomes/Vulture (voice)

9. 

   Crispin Freeman

   Max Dillon/Electro (voice)

10. 

    Jeff Bennett

    Montana/Shocker (voice)

11. 

    Daran Norris

    J. Jonah Jameson (voice)

12. 

    Joshua LeBar

    Flash Thompson (voice)

13. 

    Peter MacNicol

    Otto Octavius/Doctor Octopus (voice)

14. 

    Alanna Ubach

    Liz Allen (voice)

15. 

    Grey DeLisle

    Sally Avril (voice), Betty Brant (voice)

16. 

    Clancy Brown

    Alex O'Hirn/Rhino (voice)

Kru 8