Wie is de Mol? is een avontuurlijk spelprogramma waarbij een team van bekende Nederlanders opdrachten uitvoert en jaagt op een mol in haar midden. De opdrachten zijn spectaculair, angstaanjagend of ontroerend, maar altijd intrigerend. Nodig zijn: inventiviteit, stressbestendigheid, intelligentie, inzicht, lef en bovenal mensenkennis.

Players working as a team complete a series of difficult physical and psychological tests, each worth a set amount of money. One of the players, however, is a "Mole" or saboteur, whose goal is to foil the efforts of the other players without revealing his or her identity. At the end of each episode, the group is given a quiz on The Mole's identity. The player who knows the least information about The Mole is then immediately sent home. In the final episode, The Mole is revealed and the one remaining player wins the jackpot, up to $1,000,000.