为研发新药，一个猎人（威廉达福饰演）受红叶医药公司委托，踏上了捕获濒临绝灭的物种塔斯马尼亚虎的旅程，寄宿在壮阔的塔斯马尼亚平原上一个男主人外出失踪半年的三口之家中。

　　猎人在苦苦搜猎之余，和女主人还有两个小孩渐生情愫，并目睹了塔斯马尼亚平原严重的环保危局，揭开了男主人失踪的迷局，发现了塔斯马尼亚虎的踪迹，更由此推断出了幕后的种种利益关联，于是，受雇的猎人就此停止狩猎，开始用心在孤儿寡母身上。

　　幕后的利益集团恼羞成怒，派出了新的猎手，猎人和一家三口，还有濒危的塔斯马尼亚虎将会怎样呢？

　　两对情侣——迈特（Leigh Whannell 饰）和尼娜（Mirrah Foulkes 饰）、杰克（Nathan Phillips 饰）和丽贝卡（Melanie Vallejo 饰）结伴来到澳大利亚的塔斯马尼亚岛。这里在18世纪到19世纪间，曾是大英帝国用来流放犯人的小岛，岛上条件恶劣，难以逃脱，除了一个名叫亚历山大•皮尔斯（Peter Docker 饰）的爱尔兰男人。

　　尼娜的姐姐曾来到这里寻找灭绝已久的塔斯马尼亚虎，但姐姐最后竟神秘死亡。8年后同样攻读生物学的尼娜来到此地，完成姐姐未竟的心愿，并试图解开姐姐死亡的谜团……

‎在澳大利亚，突然出现了一个奇怪的类人马，一位研究这些生物的社会学家爱上了一个女性。这是危险的组合吗？‎

Recorded by pioneers as far back as 1805, the Tasmanian tiger has become an intensely mystifying Australian icon, whose entire existence has become the stuff of both fable and legend. This program investigates a chequered past and puts the speculation into perspective, taking into account the tragic culling and ‘bounty era’ where the carnivorous creatures were thought to be solely responsible for a considerable loss of farmers’ livestock. Balancing the facts with personal reflections from Tasmanian locals, scientists and other informed practitioners, The Tasmanian Tiger is a thought-provoking and revealing look at the extraordinary life and death of one of Australia’s most mysterious marsupials.

A Tasmanian tiger wanders around in his zoo enclosure. A glacier is slowly melting. Facing its predicted disappearance, nature exerts its fury, bursts over the frame and resists its extinction by transformation.

A trio of experts venture into Tasmania's undeveloped wilderness in search of the Tasmanian Tiger, one of the most terrifying predators ever to walk the earth.