As Batwoman continues to keep the streets of Gotham safe, Batwing joins in on the action, but Luke quickly realizes he hasn't quite mastered his suit. When Ryan pays Alice a visit to ask about the bombshell she dropped, Ryan must decide if she should go down the rabbit hole of her past. As Mary prepares to finally graduate from medical school, she feels the absence of her family more than ever. When an Alice admirer stumbles upon one of the missing Bat Trophies, Gotham and the Bat Team get mixed up in the madness, culminating in a shockingly gruesome graduation... and an equally shocking new partnership.

Ryan asks Sophie to accompany her to a dinner at Jada’s to do recon on what her estranged mother knows about Wayne Enterprises, but the evening takes a turn when an uninvited guest drops in on the intimate gathering. As the terrifying evening unfolds, Ryan witnesses another side to Marquis and her mother.

An unnerving discovery in a garden shed sends Renee Montoya rushing to Ryan to ensure the city is on high alert. Her terrifying theory — a Bat Trophy has entangled a new host, and her thorny ways are weaving their way through Gotham. Alice is suspiciously eager to help the Bat Team, and Sophie seeks vengeance for an incident involving her sister.

While Mary and Alice continue their sisterly bonding, Ryan, Luke and Sophie are desperate and willing to do almost anything to stop them. Renee is forced to revisit her heartbreaking past. Meanwhile, Marquis grows more unhinged and throws a spectacularly sinister soiree at Wayne Tower... and everyone is invited.

Ryan, Sophie and Luke attempt to track down the OG Poison Ivy when all signs point to Pamela Isley’s reappearance in Gotham. At the same time, Mary feels drawn by a powerful force and Alice suddenly feels very protective of her stepsister. Meanwhile, Jada hasn't given up on rehabilitating Marquis and seeks the help of an old friend.

Batwoman’s hands are full as she juggles Jada’s demands, Mary’s ever-increasing power and a new romantic entanglement. Loyalties are tested and motives come into question as Batwoman races against the clock to once again save the city and those closest to her.