A Chuck Norris fan (Jason Sudeikis) sings a rock ballad about the action star (Samberg) before he became famous. There are cameos by Fred Armisen as a criminal mugging a woman, played by Amy Poehler, and Taccone as a man being robbed by Bill Hader.

New office employee Gary (Bill Hader) gets into a fight to the death with incumbent cubicle holder Steve (episode host Dane Cook). Jason Sudeikis appears as the boss and Andy Samberg, Will Forte, Fred Armisen, Kenan Thompson, Amy Poehler and Kristen Wiig are other employees cheering on the fight.

A corporate executive (Rainn Wilson) leads a meeting to take ideas on how to save his failing company, and hears suggestions from an increasingly bizarre set of employees, including a gigantic turkey sub and Arcade Fire. Upon reaching the end of the meeting, Wilson receives a phone call, seemingly informing him that the office building is about to be blown up. Then, the office building explodes, killing all of them inside.

In a spoof of The O.C. episode "The Dearly Beloved", a man (Hader) writes a letter to his sister, he is shot by his friend (Samberg), leading to a series of overly dramatic, slow-motion shootings set to "Hide and Seek" by Imogen Heap, including their roommate (Shia LaBeouf), the sister (Wiig), and two police officers (Sudeikis, Armisen).

In a style similar to the Season 32 Digital Short Andy Popping Into Frame, Samberg sneaks up on people and punches them just as they area about to start eating their respective foods, then gleefully dances afterwards. His victims include Forte, Armisen, Sudeikis, Taccone, episode host Jon Bon Jovi, and Taylor Hawkins and Dave Grohl of the Foo Fighters. The victims, who have turned into zombies, show up en masse and chase Samberg around the world before breaking out into the Zombie Dance. The shorts ends with a close-up shot of Samberg's face above a graphic that reads, "Believe in Your Dreams".

A girl (Ellen Page) wakes up from a nightmare and begins seeing a zombie (Samberg) in her bathroom mirror, which turns out to be the nightmare of the zombie, a wolf man (Forte), and a woman named Debbie (Wiig) who is married to Dracula (Sudeikis). After several nightmares, the camera returns to Page waking up from a nightmare, and as the short ends, the zombie pops up at her bedside.

An apparently wealthy man (Samberg) sings about crime infecting the city, then turns into a superhero (à la Batman) and tries to save a woman (Amy Adams) from being mugged, only to have the mugger (Sudeikis) beat him to death.

Samberg and host Shia LaBeouf sing a high-energy country song for an infomercial about Samberg's new dress shirt, black socks, no pants look (the best look in the world). Armisen, Forte, Thompson, Sudeikis, and Hader all appear as people following the trend (a dad, a husband, two cops, and an illiterate genie).

Ricky Gervais presents a clip from a Japanese show that was his inspiration for The Office, featuring Japanese versions of Michael (Steve Carell), Dwight (Hader), Jim (Sudeikis), Pam (Wiig), and Stanley (Thompson). Also, Darrell Hammond plays Regis Philbin in a tampon commercial.

At the end of the episode, Gervais comments "It's funny 'cause it's racist".

Samberg, dressed in a white pompadour wig and spangled jumpsuit, sings a song promoting a low-budget, ill-planned, ultimately doomed athletic competition held in space in the year 3022. Athletes are portrayed by Sudeikis, Casey Wilson, Hader, and episode host Michael Phelps.

A sequel to the "Sloths" video, only with Jason Sudeikis as the head of the Staten Island Zoo instead of Kristen Wiig. The same students create a video that proclaims giraffes are from outer space and will destroy mankind.

A corporate executive (Hugh Laurie) announces to the department heads that things are not going well and drastic steps may need to be taken. As the others ask questions and fret about their futures, Marcus (Armisen) becomes increasingly distracting as he stretches himself out to reach a plate of cookies on the table. At the end of the skit, another executive (Thompson) reveals that the "cookies" are really laxatives.

Neil Patrick Harris and the entire SNL cast perform the theme to Doogie Howser, M.D.. Harris wears a tuxedo while everyone else is dressed as Doogie Howser in blonde wigs, lab coats with a picture ID of Doogie on them, dress shirts, colorful ties, acid washed jeans, and Nike hi-tops (Armisen and Samberg wear green sunglasses as well). Harris plays keyboard; Wilson, Elliott, and Michaela Watkins play violin; Moynihan plays tuba; Thompson plays harp; Hader plays saxophone; Hammond plays trumpet; Wiig plays electric guitar; Sudeikis plays double bass; Forte plays drum kit; Armisen plays synthesizer; and Samberg plays an Akai MPC 2000XL drum machine. At the end of the performance, Harris sheds a single tear.

During a performance review conducted by episode host Seth Rogen, Samberg raps about his typical day as the boss of a large company. The first few activities he names are what one might expect, such as approving memos and micromanaging his staff (John Mulaney). His actions become increasingly erratic, however, after a subordinate (Kristin Wiig) rejects his advances and he is consequently denied a promotion. The boss's description ends with him turning into a jet, crashing into the sun, and dying. Rogen leaves the performance review in apparent disgust as Samberg repeatedly reminds him, "I'm the boss."

The fifth single from The Lonely Island's debut album, Incredibad, Rogen's voice replaces Schaffer's as the company evaluator, though Schaffer does appear in the short as a hooded gun dealer. Taccone also makes a cameo appearance as the man who hands Samberg a notice about a harassment lawsuit filed by Wiig.

Saturday Night Live celebrates 40 years in a star-studded 3-and-a-half hour live special featuring appearances from former and current cast members, past hosts and guest stars.