The Batman legend takes an unexpected turn when the Caped Crusader vanishes from the crime-ridden city of New Gotham and his legacy is taken over by a trio of beautiful and relentless heroines - the Birds of Prey. Barbara Gordon started out as Batman's protege, Batgirl, but an attack by The Joker left her confined to a wheelchair. Reinventing herself as Oracle, she takes under her wing Helena Kyle, the secret daughter of Batman and Catwoman, who quickly grows into the fierce and beautiful "Huntress", and Dinah, a teenage runaway who is drawn to the city by meta-human visions. With the help of the only honest cop in New Gotham, Detective Jesse Reese, the Birds of Prey fight their first battle against a mysterious madwoman who is bent on their destruction.

Slick, an evil meta-human who can turn from solid to liquid, is hired by Dr. Quinzel to kill the honest cops who stand in her way. When Reese is the next intended victim, Helena comes to his aid and their mutual attraction continues to grow. Meanwhile, Barbara believes Dinah's story that she's just a runaway, but Helena remains suspicious.

At the request of Det. Reese, Helena goes undercover to help bring down a gang of thieves terrorizing New Gotham and quickly discovers that the gang is part of a larger infrastructure. Helena goes after the big boss until her cover is blown, putting her in grave danger. Meanwhile, Barbara has to deal with her fear of intimacy when her relationship with Wade takes a serious turn.