The second semi-final took place on 13 February 2016 at the Malmö Arena in Malmö, hosted by Gina Dirawi. Charlotte Perrelli was originally supposed to co-host the show; however, after a breach of contract arose due to her endorsement deal with mobile phone brand Comviq, she appeared only as a guest performer instead. "We Are Your Tomorrow" performed by David Lindgren and "Save Me" performed by Wiktoria qualified directly to the final, while "Hunger" performed by Molly Pettersson Hammar and "I Will Wait" performed by Isa advanced to the Second Chance round. A total of 4,647,934 votes were cast by the viewers during the show with a total of 487,371 SEK collected for Radiohjälpen.

In addition to the performances of the competing entries, Dirawi and Perrelli opened the show with a performance titled "Flickorna från Sverige" (English: The girls from Sweden), while the interval act titled "Rädda schlagerdivan" (English: Save the schlager diva) included a video presentation that featured Claes Elfsberg and Arja Saijonmaa followed by Perrelli performing a Swedish language cover of the song "Atemlos durch die Nacht" by Helene Fischer entitled "Här står jag" (English: Here I stand). Recurring interval acts such as Jonas Gardell's schlager school and dramatic readings of past Melodifestivalen song lyrics by Swedish actors were also part of the program; Krister Henriksson and Peter Haber performed a dramatic reading of Kikki, Bettan and Lotta's Melodifestivalen 2002 entry "Vem é dé du vill ha".