Season 1 of Holby City premiered on January 12, 1999.

Season 2 of Holby City premiered on November 25, 1999.

Season 3 of Holby City premiered on October 5, 2000.

Season 4 of Holby City premiered on October 9, 2001.

Season 5 of Holby City premiered on October 8, 2002.

Season 6 of Holby City premiered on October 7, 2003.

Season 7 of Holby City premiered on October 19, 2004.

Season 8 of Holby City premiered on October 18, 2005.

Season 9 of Holby City premiered on October 24, 2006.

Season 10 of Holby City premiered on October 16, 2007.

Season 11 of Holby City premiered on October 21, 2008.

Season 12 of Holby City premiered on October 20, 2009.

Season 13 of Holby City premiered on October 19, 2010.

Season 14 of Holby City premiered on October 18, 2011.

Season 15 of Holby City premiered on October 16, 2012.

Season 16 of Holby City premiered on October 15, 2013.

Season 17 of Holby City premiered on October 14, 2014.

Season 18 of Holby City premiered on October 13, 2015.

Season 19 of Holby City premiered on October 11, 2016.

Season 20 of Holby City premiered on January 2, 2018.

Season 21 of Holby City premiered on January 2, 2019.

Season 22 of Holby City premiered on January 7, 2020.

Season 23 of Holby City premiered on April 6, 2021.