Cast 20

1. 

   Ben Diskin

   Nigel "Numbuh One" Uno / Hoagie Pennywhistle "Numbuh Two" Gilligan Jr. / D.C.F.D.T.L. (Voice)

2. 

   Lauren Tom

   Kuki "Numbuh Three" Sanban (Voice)

3. 

   Dee Bradley Baker

   Wallaby "Numbuh Four" Beatles / D.C.F.D.T.L. / Chip / Toiletnator / Mr. Fibb (Voice)

4. 

   Cree Summer

   Abigail "Numbuh Five" Lincoln / D.C.F.D.T.L. (Voice)

5. 

   Maurice LaMarche

   Father (Voice)

6. Ric Stoneback

   Adult Hoagie

7. Sonnie Brown

   Adult Kuki

8. 

   Jeff Bennett

   Mr. Boss (Voice)

9. 

   Jennifer Hale

   Computer / Mrs. Uno / Numbuh 86 / Numbuh 363 (Voice)

10. 

    Mark Hamill

    Stickybeard (Voice)

11. 

    Janice Kawaye

    Sonia / Lee (Voice)

12. 

    Tom Kenny

    Mr. Dink / Knightbrace (Voice)

13. 

    Rachael MacFarlane

    Numbuh 362 (Voice)

14. 

    Frank Welker

    Mr. Uno (uncredited) (Voice)

15. 

    Candi Milo

    Lydia Gilligan (Voice)

16. 

    James Arnold Taylor

    Nick (Voice)

17. 

    Daran Norris

    Count Spankulot (Voice)

18. 

    Rob Paulsen

    Robin Food (Voice)

19. 

    Fred Tatasciore

    Cheese Shogun Roquefort (Voice)

20. 

    Dave Wittenberg

    Numbuh 74.239 (Voice)

Crew 2