As Batwoman continues to keep the streets of Gotham safe, Batwing joins in on the action, but Luke quickly realizes he hasn't quite mastered his suit. When Ryan pays Alice a visit to ask about the bombshell she dropped, Ryan must decide if she should go down the rabbit hole of her past. As Mary prepares to finally graduate from medical school, she feels the absence of her family more than ever. When an Alice admirer stumbles upon one of the missing Bat Trophies, Gotham and the Bat Team get mixed up in the madness, culminating in a shockingly gruesome graduation... and an equally shocking new partnership.

When a new incarnation of Gotham's swamp slumming Killer Croc surfaces and starts racking up a body count, the inaugural team-up of Batwoman and Alice is put to the test. But Alice isn't the only unwanted intrusion into Ryan's life when Jada Jet shows up at Wayne Enterprises, insisting on meeting the company's new CEO. Luke and Mary's relationship is strained under the weight of a secret Luke is keeping, while Sophie finds herself becoming part of Ryan's inner circle.

An unfortunate incident in downtown Gotham alerts Batwoman and the Bat Team that another missing trophy has made its way into the wrong hands. Meanwhile, a new member of the Jet family surfaces when Marquis Jet makes a not-so-subtle entrance, interrupting a very personal moment between Ryan and Jada. Batwing must decide if he’s ready to suit up again, and the Hippocratic Oath forces a strained sisterly moment between Alice and Mary.

When Ryan makes Gotham’s “30 Under 30” list, Luke worries about how Marquis's involvement in Ryan’s rising popularity as Wayne Enterprises’ acting CEO will land with Jada. Mary returns from a late night out not feeling quite herself, while Alice’s hallucinations grow stronger. And when Sophie’s sister, Jordan, goes missing, she and the Bat Team quickly realize that Freeze’s missing weapon hasn’t been fully contained.

Ryan asks Sophie to accompany her to a dinner at Jada’s to do recon on what her estranged mother knows about Wayne Enterprises, but the evening takes a turn when an uninvited guest drops in on the intimate gathering. As the terrifying evening unfolds, Ryan witnesses another side to Marquis and her mother.

An unnerving discovery in a garden shed sends Renee Montoya rushing to Ryan to ensure the city is on high alert. Her terrifying theory — a Bat Trophy has entangled a new host, and her thorny ways are weaving their way through Gotham. Alice is suspiciously eager to help the Bat Team, and Sophie seeks vengeance for an incident involving her sister.

As Ryan’s family dynamic grows more complicated, she also finds herself in the middle of a Bat Team stand-off between Luke and Mary. Meanwhile, Alice finds a new sidekick to do her bidding.

While Mary and Alice continue their sisterly bonding, Ryan, Luke and Sophie are desperate and willing to do almost anything to stop them. Renee is forced to revisit her heartbreaking past. Meanwhile, Marquis grows more unhinged and throws a spectacularly sinister soiree at Wayne Tower... and everyone is invited.

Ryan, Sophie and Luke attempt to track down the OG Poison Ivy when all signs point to Pamela Isley’s reappearance in Gotham. At the same time, Mary feels drawn by a powerful force and Alice suddenly feels very protective of her stepsister. Meanwhile, Jada hasn't given up on rehabilitating Marquis and seeks the help of an old friend.

Batwoman’s hands are full as she juggles Jada’s demands, Mary’s ever-increasing power and a new romantic entanglement. Loyalties are tested and motives come into question as Batwoman races against the clock to once again save the city and those closest to her.

Just when it seems things can’t get worse... Batwoman’s identity is once again at risk of being exposed, and some of Gotham’s most villainous unite. Sophie joins Luke on a mission and Alice gets into Mary’s head.

Ryan, Sophie y Jada trazan un plan para detener a Marquis, mientras Mary intenta arreglar las cosas tras el daño causado como Hiedra Venenosa. Alice está fuera de control y sigue enfrentándose a sus demonios.

En el episodio final de la tercera temporada, Batwoman y el Bat equipo trabajan contra reloj cuando los terribles planes de Marquis se convierten en algo que Gotham ni sospecha ni ve venir.