Shinpachi Shimura returns to the Yorozuya and discovers his friends drastically changed during the break. Feeling left behind, he returns home and discovers his sister, Tae Shimura, engaged to Isao Kondo. Tae and Kando enlists Shinpachi into the Shinsengumi and reveals that Tae is pregnant. Shinpachi runs to the ocean and laments the sudden change of those around him. There, he learns about Kyubei Yagyu's sex change to modify her life. They are interrupted by and Kotaro Katsura who also changed his genre due to his Kyubei's characters similarities.

As Katsura and Kyubei fight for the title of the best transgendered, Shinpachi leaves to find that the Shinsengumi members also suffered a sudden change in their selves; Sogo Okita is the leader from the group and orders him and Toshiro Hijikata, Sagaru Yamazaki to attack the Yorozuya. As the three leave, Hijikata knocks out Yamazaki, as he was faking his change. He and Shinpachi learn that an invasion of parasite aliens is responsible for change to their friends and proceed to destroy them. However, they find problems in the outcome, and both Hijikata and and Shinpachi are knocked out in the process, with Gintoki being actually only person without a parasite.

As Gintoki complains of writing News Years cards, Yorozuya learn of what his friends have done over the holidays. He also receives cards saying his friends have gotten married, while they have lied and just Photoshopped the photos. The episode ends with Kondo, Sachan and Hasegawa telling their story of how they wrote a News Years card. The second part starts with the same opening, but Gin complains of the lack of chocolate. Kagura has a chocolate for Gintoki and Shinpachi, but is too embarrassed to give to them. She enlists the help of Tsukuyo, and Sachan to help, but they are each too embarrassed to help her and make her give their chocolates to Gintoki. She indifferently give Gintoki and Shipachi the chocolate saying they were on the door step, as the happily run for the chocolate, they find all the chocolates were said to be from Kagura.

Yamazaki has a mental breakdown cause of Anpans and Kondo finally gets a date (Or does he!)

Catherine appears to have a boyfriend and has drastically improved her appearance. Her boyfriend is a regular at Snack Otose, an entrepreneur by the name of Suejiro. Catherine decides to leave the Kabuki district to start a new bar with him.

The Odd Jobs' TV breaks out with Sachan inside. Gintoki tells Sadaharu to throw her away with the TV, but Sachan's precious glasses are broken in the process. Gintoki reluctantly buys Sa-chan a new pair of glasses, but they are bottle bottom glasses that are clearly the wrong prescription. Sa-chan is overjoyed that Gintoki has given her a present, but the new glasses are responsible for a chain of failures on her assassination job. Her superiors now question her commitment.

Gintoki and crew continue to defend Sarutobi against the Lethal Punishers. But against such assassins as the Pillow Masa, T-shirt Tatsu, and the Chuubert Chu Brothers, will they win?

Due to a lack of budget, the Yorozuya try to use old footage to make a filler episodes. While Hasegawa first plays a theme song, the characters then watch a fake trailer a supposed second film based on the series. The episode ends with a chapter of "Teach us Ginpachi-sensei", a story in which Gintoki is the teacher from high school class composed of anime's characters.

Pirako Chin is looking for the strongest man in the Kabuki District. Now Gintoki has an underling who wants to turn Kabuki District into a garden of bright red flowers.

Pirako has double crossed Gin! Her real plan is to help Jirocho, her father, take over the town. Can Gin stop a war?

Pirako is pleased to hear that Otose, the reason for Jirocho's attachment to the Kabuki district, has been eliminated. With Otose gone, the balance of power among the Four Devas has crumbled and a war is in the works in the Kabuki district. Meanwhile, the Odd Jobs crew is watching over Otose when Saigo appears and informs them that the bar will be destroyed by the Four Devas. He urges them to leave this town, now that they've lost their home...Finally, the day comes for them to leave Snack Otose.

Tama, Tatsumi, Tetsuko, Kozen, Madao, Kyoshiro. Everybody is out in full force to fight for the Kabuki District. Male escorts vs. trannies, Cabaret girls vs. Yakuza, robots blowing things up. It’s an all-out war!

Jirocho and Gin battle Kada's elite Harusame forces while the rest of the denizens of Kabuki-cho take on her main forces in the streets. Kada is utterly stunned when her elite forces are beat by just these two humans, and flees the scene. Gin then helps Jirocho resolve his issues regarding Tatsugoro; Otose and Jirocho reconcile at the hospital. Outside the hospital, Kada desperately launches one last feeble attempt at conquering Kabuki-cho, but is stopped in her tracks by Pirako and the Yorozuya - Kada settles for swearing that the Harusame will be back, and flees yet again. At the very end, Jirocho denounces all other names he has ever been called except for Father of Pirako, and goes with his daughter back to the country.

Kagura's older brother, Kamui, visits Kada's cell where she is being kept after having been captured by the Kiheitai and loses at a game of odds or evens. He is approached by one of the 12 captains who tells him he has the 12 captains support if he chooses to overthrow Admiral Abo. Kamui orders his second to kill the members of the Kiheitai, isolating Takasugi so Kamui can duel him. As the Kiheitai's ship is about to be attacked the Admiral double crosses Kamui and imprisons him out of fear. Takasugi visits Kada's cell and loses at a game of odds or evens. After a brief conversation Takasugi rescues Kamui at his execution, overthrowing Admiral Abo. Kamui becomes Admiral and the episode ends with Takasugi visiting Kada's cell one last time to lose at a game of odds or evens.

Kagura invites a bunch of kids to the Yorozuya. They, along with other characters get shunned by them and invites them into a "factory tour".

Gintoki and Hasegawa have to help the shogun when he is at the pool

Otose sent a box of crab to the Yoruzuya. Before they can divide the crab's legs to themselves, suddenly, there came a blackout after the electricity came back one leg is missing and Gintoki ate it. Another blackout came and another leg was missing and Kagura ate it, saying she was attacked by a gorilla. Shinpachi then put up a candle. Then Gintoki and Kagura ate all of the legs. When Shinpachi claims the crab's whole body they found themselves fighting for a crab congee and ended up eating crab-flavored cheap food.

Kagura helps a iwamatsu, the last part have the yoruzuya and shinsengumi in the bathhouse just as neighbor Hedero arrives with family members visiting Edo.

Gin, Kondo, Hijkata, and Shin are stuck in the public bath with the demons. Every lie they tell digs their grave deeper. Can they really get away with kicking heads in, ripping off horns, and setting demons on fire with lotion?

The monkey, aka "Jugem Jugem... (abbreviated) Runny Diarrhea," has grown close to Kyubei. However, their time together did not last. The Shogun family ordered that the Yagyu return Runny Diarrhea. The monkey escapes from the Shogun family to see Kyubei again. Kyubei and the other Yagyu members are forced to continue a desperate and fruitless search for Runny Diarrhea.

The monkey, aka "Jugem Jugem... (abbreviated) Runny Diarrhea," has grown close to Kyubei. However, their time together did not last. The Shogun family ordered that the Yagyu return Runny Diarrhea. The monkey escapes from the Shogun family to see Kyubei again. Kyubei and the other Yagyu members are forced to continue a desperate and fruitless search for Runny Diarrhea.

Katsura infiltrates Matsudaira's household and ends up pondering family values.

The Yorozuya run into a mysterious Blu-ray DVD with a depressed female ghost in it. The ghost moves into their TV, making all the programs gloomy as well. Gintoki then attempts to find use for the ghost as a navigator.

Gintoki gets thrown in prison after being framed, and while he's made an enemy of the warden, he's managed to become the boss of the prisoners. With his underling Shachi in tow, Gintoki skips his menial labor duties to search the warden's room for anything incriminating. Understanding that the punishment will be fatal if they are caught, Gintoki disguises himself by putting on pajamas...

Gintoki continues creating havoc in the prison.

Gintama meets Sket Dance! But not without trouble...

Shinpachi introduces his new girlfriend to Otae...but she turns out to be Momo-san, a character from the popular Love Choris game. There are more and more boys in Edo who can no longer distinguish game from reality like Shinpachi. Otae asks Gintoki to bring Shinpachi back to the real world. The only way is by entering the world of Love Choris and talking some sense into him. Gintoki ends up choosing a very unique heroine to be his girlfriend...

Gintoki challenges Shinpachi in the ultimate Love Chorus tournament in order to break his illusion of his virtual girlfriend.

The Shinsengumi and the Yorozuya attend the funeral of a close udon shop owner. Instead of moving on, however, the soul of the old man is overseeing the ceremony.

Katsura visit Yorozuya with a Elizabeth's placard to solve the mystery of Elizabeth's disappearance. But the investigation only reveals a shocking truth.

The Yorozuya and Katsura follow Elizabeth to stop the Renho from taking over the earth.

The Yorozuya, Katsura, and Sakamoto disguise themselves as Renho and infiltrate their base.

Elizabeth and Dark Vader battle to the death and just when things are not looking so good for Elizabeth, Katsura shows up to save his friend.

The Renho join forces with Sakamoto and the Yorozuya against Dark Vader and their home planet in order to bring peace to the universe.

The Shinsengumi are forced to guard the Shogun on his first snowboarding trip, but they run into the Yorozuya and mayhem ensues.

Everybody's stranded in the middle of the mountains. To make things worse, the Shogun goes missing.

It's a year-end party at Odd Jobs! The Gintama anime has been through a lot, but Gintoki tells Kagura and Shinpachi to keep it cool. And then the usual members join in for some real partying... The next morning, Gintoki wakes up to discover that he's made some very bad mistakes.

After getting drunk at the year-end party, Gintoki managed to become a six-timer in one night. In order to prevent the show from being pulled off the air, Gintoki is forced into engaging in a relationship with all of them. And now he has to go on dates with all of them at the same time...

The number one host in the Kabuki district, Kyoshiro, summons the Odd Jobs members to his club, Takamagahara, and tells them that he intends to quit because of a lady named Madame Yagami who controls the night world. It seems that she declared she would be making a second visit to Takamagahara, which is essentially a death sentence. The hosts at Kyoshiro's have quit out of fear, so Gintoki and co must find hosts to handle the Madame...

The Madame's arrival is imminent. Kyoshiro, Gintoki, and friends attempt to scout some help, but they end up with a bunch of oddballs. And it turns out that Otae has invited guests from all over town who go wild and leave the bar and Kyoshiro broken! There's no time to worry about taking care of customers.

Jellybeans Vanguard Honda is on his fifth year as a Jump editor. However, he's been assigned to the manga Gintaman, which has driven multiple editors insane. In order to save himself, Honda must find a new talented artist so he can be reassigned. One day, he is visited by an aspiring manga artist by the name of N3016.

The Sasaki family is as elite as it gets, and one of its members, Tetsunosuke, has joined the Shinsengumi. He would have joined the Mimawarigumi, a police force with pedigree, but he is considered a troublemaker, which is why he was sent to the Shinsengumi. Kondo assigns Tetsu to Hijikata as an assistant, so Hijikata is forced to endure the new recruit...

Tetsunosuke has completely changed as he sincerely tries to perform his new job. Isaburo despises Tetsunosuke and tells Hijikata that he should dispose of him, but Hijikata, who came from a similar background, sympathizes with Tetsunosuke and looks after him. Meanwhile, Gintoki, who was arrested by the Mimawarigumi, has been assigned a mission by Isaburo...

Tetsunosuke has been ordered to deliver a letter for Hijikata. However, he's captured by the radical Joi Check It Out gang in the process. Tetsunosuke had concealed his identity to join the gang, but now they know that he has connections to the police. He's taken hostage as leverage over the Shinsengumi and Mimawarigumi. However, the Mimawarigumi shows no concern for the hostage and plots to place the blame squarely on the Shinsengumi. Hijikata and the other members are enraged after learning Isaburo's plans. The Shinsengumi and Mimawarigumi are now in an all-out fight.

The Shinsengumi attempt to save Tetsunosuke from the Check It Out gang, but the Mimawarigumi stands in the way. Neither side is willing to back down, but Gintoki, who had infiltrated the gang as a Mimawarigumi spy, manages to save Tetsunosuke! Kondo orders the Shinsengumi to begin fighting back!

The middle-aged dumb-ass oldie, or Madao, Taizo Hasegawa finds himself on a game show with a chance to win ten million yen. Will he be able to endure the pressure and become a Madaonaire!? And what is Madao's motivation for participating in the game show...?

A character from 2 years ago is back with a request for Gin. This favor will take the Odd-Job Trio right in the middle of Satan Festival in this very special Christmas episode.

Gin is trying to hand out New Year Envelopes but the lack of children characters make it hard. To make matters worse, everyone seems to have their mind wrapped around long cylindrical objects and small spherical objects.

Nobody cares anymore, the animators, producers, not even Hideaki Sorachi. Aww, it’s such a drag even the characters are airing their grievances. Where do we go from here?

Its last episode and Gintama' is going out with a bang. A Gintama clip show!