Nina meets with an old friend and boss, Mister Rosso. The delightful old boss however, has a very dark past.

Suspecting foul play in Robert's 'accidental' death fall, Dr. Reichwen makes some discoveries in his investigations. But as he later realizes, his investigations may have put him in harms way.

Dr. Reichwein reveals the true nature of Johan to Mr. Schuwald. Dr. Tenma gets ready to assassinate Johan. Lunge concludes that Dr. Tenma will kill Schuwald next. Lotte confronts Nina about Johan.

Schubert, with the help of Reichwein, re-explores his past with Karl Neumann's real mother and realizes his link to Johan. As news of Tenma's arrest spreads, various people react with shock and horror, with the exception of Eva Heinemann, who is insistent that Tenma pay dearly for rejecting her. Suk tries to leave the hospital he is in to try to help Tenma, but is stopped by the Prague Police who inform him that Grimmer has sent a note to them exonerating Suk from any involvement of the deaths of the police officers. Runge also gets news of the arrest as well, but he is more interested in the Mansion of Red Roses, where he obtains a letter possibly addressed to the twins' mother. Tenma is deported back to Dusseldorf.

Nina and Dieter enters the Red Rose Mansion, but Nina faints from her disturbing experience. And they end up in the care of a puppeteer name Lipsky.

A flashback to Johan’s and Nina’s past, to the time before the Liebert’s homicides reveals the reason behind Johan’s killing. Police makes a startling discovery at the burnt down mansion. Tenma finally learns where Eva is. And Lunge reveals Lipsky’s true identity.

Capek tries to berate Johan for his actions and tells him that everything should be done according to Capek's plans but Johan simply disagrees and disappears. Capek realizes his hold on his organization is crumbling and has his men pick up Nina in Frankfurt. Eva accosts the young man who was introduced to Johan, Christof Sievernich, demanding answers.

Nina confronts Johan who presents her with a story on what happened when many people were poisoned at the Mansion of Red Roses. Nina realizes that she was the one who witnessed these deaths, the shock of this revelation nearly drives her to suicide, but she is saved by Tenma. Capek is gunned down by members of his organization when they find out about the guard that Capek killed.

Lunge confronts Roberto, one of the ringleaders, in a hotel room. Both are subsequently wounded in the fight. Nina and Gillen arrive in Ruhenheim where they find a house containing drawings of the two twins by Bonaparta. This triggers a final memory and the secret to Johan's madness and motives, his quest for true solitude, as well as forgiveness for his actions. Bonaparta accompanies Tenma on his quest to find and kill Johan.

Bonaparta attempts to shoot Johan dead, but is gunned down by Roberto, who dies of his own injuries soon afterwards. With the massacre now over, authorities rush into the town and attempt to determine what happened. Just as an officer is about to arrest Dr. Tenma, an ambulance worker arrives asking for Tenma's assistance.