Something odd is happening - vampires seem to be growing in number. And when an Italian college student is found to have a computer chip, which has apparently made him into an 'artificial' vampire, it raises more than a few eyebrows. Even though the chip is removed, the student revives, killing many on his search for his lover, who he was caught in the act of making one of his own. Hellsing moves to put a stop to this, but there seems to be more than one person interested in the boy.

Hellsing's agents are killed, and Seras is severely wounded. Not by the student, but by a man called Alexander Anderson, a member of the Vatican's section 13 - the Iscariot organization. Though having the same mission as Hellsing, the two organizations are sworn enemies, and when Arucard shows up, a heated battle takes place. One would think a human would be no match for the likes of a vampire, Anderson proves to have more than one trick up his sleeve.

More than half of the members of the Royal Order of Religious Knights were massacred due to the last attack on them that was conducted by the Valentine Brothers. Sir Integra Hellsing, Arucard, and Seras must now hunt down the perpetrators. The suspects include the Vatican's Iscariot Organization. Arucard must do battle once again with the notorious Alexander Anderson.