A Chuck Norris fan (Jason Sudeikis) sings a rock ballad about the action star (Samberg) before he became famous. There are cameos by Fred Armisen as a criminal mugging a woman, played by Amy Poehler, and Taccone as a man being robbed by Bill Hader.

In a frame story, Hader and Samberg pitch their new ultra low-budget Digital Short, Laser Cats! to SNL executive producer Lorne Michaels. In the Laser Cats! short itself (a short within a short), Hader and Samberg play the heroes Nitro and Admiral Spaceship in a post-nuclear war world in which cats can shoot lasers from their mouths and are used as weapons. They are saving the princess (Lindsay Lohan) from the evil Robotron (Will Forte). Rachel Dratch makes an appearance, disguising herself as the princess/Lohan. Jorma Taccone appears in the opening credits for Laser Cats!. Fred Armisen also makes a cameo.

Laser Cats! is shot in the SNL offices with gleeful haphazardness because, according to Samberg in the special Saturday Night Live in the 2000s, “it’s supposed to suck.”

Harpoon Man (John C. Reilly), a suave action hero and parody of Shaft, tracks down an insulting announcer dressed as a whale (Samberg), who is narrating his life in a theme song. There are appearances by Jorma Taccone, who plays a man getting robbed, and Bill Hader, who plays the man robbing Taccone.

In the same frame-story format, Hader and Samberg apologize to Lorne Michaels for the original Laser Cats! claiming to understand where they went wrong: not enough politics. They then introduce Laser Cats! 2, based on the same premise, now set in the Iraq War which has gone nuclear and caused the feline mutations. This time, Dr. Scientist (Jake Gyllenhaal) has stolen the cure that turns Laser Cats back into regular cats. Jorma Taccone appears in the opening credits for Laser Cats! 2 (Which is the same as Laser Cats!), and Fred Armisen and Amy Poehler are the scientists who created the cure.

In a style similar to the Season 32 Digital Short Andy Popping Into Frame, Samberg sneaks up on people and punches them just as they area about to start eating their respective foods, then gleefully dances afterwards. His victims include Forte, Armisen, Sudeikis, Taccone, episode host Jon Bon Jovi, and Taylor Hawkins and Dave Grohl of the Foo Fighters. The victims, who have turned into zombies, show up en masse and chase Samberg around the world before breaking out into the Zombie Dance. The shorts ends with a close-up shot of Samberg's face above a graphic that reads, "Believe in Your Dreams".

Samberg and Taccone rap about their personal experiences with premature ejaculation, often at the slightest provocation. Schaffer plays the role of a DJ who mixes beats for the other two as they perform. Molly Sims, Jamie-Lynn Sigler, and Justin Timberlake make cameo appearances.

The third single from The Lonely Island's debut album, Incredibad, and the first music video featuring all three members.

Samberg wins a prize from a cereal box for a boat trip for three people and selects Schaffer and, to Taccone's surprise and dismay, T-Pain to join him. The short then cuts to the lucky trio performing an "aggressive" and expletive-laden rap about their boat ride (the word "fuck" is used 17 times), with occasional shots of Taccone having a miserable time back on land.

The fourth single from The Lonely Island's debut album, Incredibad.

During a performance review conducted by episode host Seth Rogen, Samberg raps about his typical day as the boss of a large company. The first few activities he names are what one might expect, such as approving memos and micromanaging his staff (John Mulaney). His actions become increasingly erratic, however, after a subordinate (Kristin Wiig) rejects his advances and he is consequently denied a promotion. The boss's description ends with him turning into a jet, crashing into the sun, and dying. Rogen leaves the performance review in apparent disgust as Samberg repeatedly reminds him, "I'm the boss."

The fifth single from The Lonely Island's debut album, Incredibad, Rogen's voice replaces Schaffer's as the company evaluator, though Schaffer does appear in the short as a hooded gun dealer. Taccone also makes a cameo appearance as the man who hands Samberg a notice about a harassment lawsuit filed by Wiig.

An unemployed cocaine addict named Dennis (Samberg) goes on drug binge while singing an elaborately choreographed Disneyesque musical-theater number about how it is going to be a great day. The more cocaine he snorts, the faster he sings, and the more disjointed and surreal the song becomes. Eventually the whole experience is revealed to be a vivid, cocaine-induced hallucination. 9th music video on Lonely Island's album, Turtleneck & Chain.

Andy Samberg, Jorma Taccone, and rapper Akon celebrate just having had sex with women (Blake Lively and Jessica Alba respectively), despite the fact that the women found the sex to be mediocre. Lonely Island's Akiva Schaffer shows up at various points during the video congratulating them on having sex, he also mouths the chorus at the end of the song.

The first single off the Lonely Island's second album, Turtleneck and Chain.

Samberg, Jorma Taccone, and Pharrell sing about how their hugs are the best but they won't lead to any kind of romantic commitment. Tatiana Maslany and Maya Rudolph (as Oprah Winfrey) make cameo appearances, as does Akiva Schaffer (who does not have any vocals on this track).

This video was included on the DVD accompanying The Lonely Island's third album, The Wack Album.