Two brothers, Sam and Dean Winchester, witness their mother's paranormal death as children and grow up trained to fight by a distraught father who wants nothing more then to hunt down the thing that killed his wife. Sam escapes to college to start a new, normal life, but gets pulled back in after Dean shows up on his doorstep to tell him their father is missing.

Following clues from an eerie phone message from him, the boys travel to a small town and encounter a vengeful spirit called the "Woman in White" who then starts to haunt Sam.

Sam is haunted by a vision of a woman trapped in his childhood house and convinces a reluctant Dean that they need to go home. Upon arrival, they meet the woman in Sam's premonition and she reveals that the house is haunted. Looking into their personal history, the brothers discover their father had visited a psychic after their mother's death and they turn to the same woman for answers of their own. Together they discover a malevolent poltergeist has inhabited the house and set out to exorcise it, but are blindsided when another unseen spirit steps in.

Sam and Dean finally make contact with their father, but he tells them to stop looking for him and sends them on another ghost-hunting job. Frustrated with their father's evasiveness, Sam sets out alone to find him while Dean stays back to obey his father. Dean investigates a small town in Indiana where couples have gone missing the same day each year and discovers the farmers are sacrificing them to a Pagan God who takes the form of a scarecrow.

Sam has a reunion with a girl he once met, Meg, but it turns out that she is using the brothers to lure their father John to his doom.

Sam and Dean battle a Shtriga, a witch that sneaks into childrens' bedrooms and sucks the lives out of them. Dean must relive his childhood when he remembers that he encountered the Shtriga years ago.

To obtain a mystic gun capable of destroying the demon that killed their mother, Sam and Dean must team up with their father John and face off against vampires.

John has realized where the demon will strike next and the Winchesters head there to stop the next killing. But Meg begins killing John's friends and demands the Colt or she'll keep going until everyone John knows is dead.

When Meg boasts that John is her prisoner, the boys visit a friend of their father's to get information about demonic possessions and ways to trap a demon. It turns out Meg is not what she appears.

In the aftermath of the car crash, the surviving Winchesters go to the hospital, where one is stalked by a Reaper while another must consider sacrificing himself to save the others.

After the fight between Jake and Sam, Dean has to make the ultimate sacrifice for his brother. Meanwhile the Yellow-Eyed Demon has his new servant and plans to unleash the hordes of Hell.

Sam and Dean search for a Crocotta that uses the phone and computer as a means to communicate with people. Its goal is to get people to commit suicide and take their soul. Dean gets some news from an unlikely source about a demon holding the contract to his soul.

Sam and Dean look to occult lore for a solution to their latest problem, but instead of a resolution, they find much more than either of them had anticipated.