The Second Chance round took place on 5 March 2016 at the Halmstad Arena in Halmstad, hosted by Gina Dirawi, Ola Salo and Peter Jöback. The eight entries that placed third and fourth in the preceding four semi-finals competed during the show in four duels. SVT decided which entries would face off in each duel. Viewers determined the results during the show through telephone, SMS and application voting. Voting in each duel commenced at the start of the first entry's performance and concluded one minute and thirty seconds following the end of the second entry's performance. Executive producer Christer Björkman announced which four entries won their duels and advanced to the final in addition to revealing the running order of the final at the conclusion of the show. "Håll om mig hårt" performed by Panetoz, "Put Your Love on Me" performed by Boris René, "Kizunguzungu" performed by SaRaha and "Bada nakna" performed by Samir and Viktor qualified to the final. A total of 6,059,917 votes were cast by the viewers during the show with a total of 441,443 SEK collected for Radiohjälpen.

In addition to the performances of the competing entries, Dirawi, Salo and Jöback opened the show with a cover of Adele's "Skyfall", while the interval acts included a duet by Salo and Jöback titled "Sing Me Out" and Hasse Andersson performing his Melodifestivalen 2015 entry "Guld och gröna skogar". Recurring interval acts that were also part of the show included Jonas Gardell's schlager school and Helena Bergström's portrayals of the characters Lasse and Marianne.