'King of the Swamp' Troy Landry teachers long time alligator hunting partner Pickle Wheat the ins and outs of Python hunting, but sometimes even the most experienced hunter's walk away with scars. Meanwhile, veteran snake hunter Bill Booth and Florida hunter Tes Lee must learn to work as a team if they hope to put a dent in the growing Python population. Zak and newcomer Aaron hunt Pythons in an ancient canal system and almost get in over their heads. Dusty Crum finds the fight for the Everglades knocking at his front door.

Pickle must face her fear of big cats as she continues to impress Troy with her natural snake hunting talent. Bill and Tes's hunt stumble on a rumored government testing facility and find all they can handle. Dusty recruits a new hunting partner with a snake phobia while Zak and Aaron battle pythons and the supernatural.

Troy and Pickle hunt a giant python known as the Night Serpent. Tess convinces Bill to try out new hunting grounds, but Bill is not sure it's a good idea. Zak and Aaron explore a notorious corner of the Everglades, and Dusty Crum has an idea to lighten his load.

Troy and Pickle witness how far the pythons have spread; Zak and Aaron take on a new type of beast; Bill and Tess tackle some uninvited guests; Dusty does what he can to prevent an early end to his python season.

Troy and Pickle face a familiar foe while Bill and Tes defend a friend's camp from a slithering invasion; Zak and Aaron's hunt leaves a bad taste in their mouths, and Dusty Crum has a plan to work smarter not harder.

Troy Landry and Pickle Wheat get a tip about a place called the snake pit from a local; Bill and Tes investigate a rock quarry full of pythons; Dusty has a close call with the supernatural, while Zak and Aaron fight to protect the local children.

The battle for the Everglades becomes personal for Troy and Pickle; Bill and Tes venture further north than ever before; Zak and Aaron visit Zak's childhood stomping grounds; Dusty heads to one of his favorite honey holes.

Hunter Bruce Mitchell joins the fight with Zak and Aaron; Troy, Pickle, Bill and Tes team up to help a rancher with a serious problem; Dusty devises a way to light up the night.

Troy and Pickle battle a hyper aggressive breed of python. Bill and Tes try to stay one step ahead of the Army Corp of Engineers. Dusty works on a plan to get the snakes to come to him, while Zak and Bruce face off against two foes.

Troy Landry and Pickle Wheat mix it up with some locals; Tes puts her skills to the test hunting without her partner, Bill; Zak and Bruce return to Viper Alley; Dusty hunts for a legend.

Troy and Pickle track a high-tech snake; Bill and Tes search for a new hot spot; Dusty discovers one of his favourite honey holes has changed forever; Zak and Bruce chase the snakes below ground and get a surprise.

Troy and Pickle help a friend look for his lost dog; Bill and Tes search for pythons in a spot ravaged by wildfires; Zak and Bruce investigate an abandoned boatyard; Dusty hunts in an area with a dark past.

Troy and Pickle help rid a citrus grove of pythons; Bill is forced to hunt solo for the day; Bruce and Zak mount an airboat to a new honey hole; Dusty responds to a friend's call for help.

The clock is ticking with only days remaining in python season; Pickle seeks validation from Troy on her tracking skills; Bruce must hunt alone in Zak's absence; Dusty heads to an isolated portion of the Everglades.

On the last day of the season, the hunters gather to target breeding females; Bill and Tes investigate a secluded stretch of islands; Dusty hopes to go out with a bang, while Zak and Bruce follow an ancient native trail.