A nuclear explosion in the far north unleashes Gamera, the legendary flying turtle, from his sleep under the ice. In his search for energy, Gamera wreaks havoc over the entire world, and it's up to the scientists, assisted by a young boy with a strange sympathic link to the monster, to put a stop to Gamera's rampage.

A ship runs aground on a mysterious atoll leading to an investigation by insurance representative Kusanagi, who discovers an ancient bead that he gives to his daughter Asagi. Meanwhile, ornithologist Nagamine investigates reports of a new species of large bird named Gyaos. As the Gyaos begin to attack, an ancient guardian with a bond to Asagi emerges.

Gamera escapes from his rocket enclosure and makes his way back to Earth as a giant opal from New Guinea is brought back to Japan. The opal is discovered to have been an egg that births a new monster called Barugon. The creature attacks the city of Osaka by emitting a destructive rainbow ray from his back, along with a freezing spray capable of incapacitating Gamera.

Unusual volcanic activity in Japan awakens Gyaos, a bloodthirsty flying monster with the power to slice things in half with an ultrasonic ray. While scientists and the military scramble to devise a way to stop this new threat, a young boy forms an alliance with Gamera; a monster no one else seems to trust.

The production of Gamera: Guardian of the Universe. Includes interviews with director Shusuke Kaneko, screenwriter Kazunori Ito and lead actress Ayako Fujitani. Also includes behind the scenes footage from special effects director Shinji Higuchi and interviews with suit actors Jun Suzuki and Yumi Kameyama.